1BHD
SECOND CALPONIN HOMOLOGY DOMAIN FROM UTROPHIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.920, 32.210, 65.360 |
| Unit cell angles | 90.00, 116.25, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1AA2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.036 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 1.990 |
| Rmerge | 0.032 | 0.125 |
| Total number of observations | 46728 * | |
| Number of reflections | 16459 * | 2296 * |
| <I/σ(I)> | 10.8 | 5.9 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 | 20 * | pH 7.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 18 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
