1BGW
TOPOISOMERASE RESIDUES 410-1202,
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 154.100, 154.100, 127.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.700 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.27700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 14.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.090 * |
| Number of reflections | 42160 |
| Completeness [%] | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 10 | 1 | reservoir | 290 (mM) | ||
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 270 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 0.1 (mM) | |
| 5 | 1 | drop | leupeptin | 0.001 (mM) | |
| 6 | 1 | drop | pepstain-A | 0.001 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 30 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 0.4 (%) | |
| 9 | 1 | reservoir | 8 (mM) |
