1BGD

CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	99.200, 36.200, 48.600
Unit cell angles	90.00, 96.40, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.207
Rwork	0.207
RMSD bond length	0.014
RMSD bond angle	2.570
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.044 *
Total number of observations	18417 *
Number of reflections	6830 *
Completeness [%]	88.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.54 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	canine G-CSF solution	22.8 (mg/ml)
2	1	drop	ethanol	5.80 (%(v/v))
3	1	drop	HEPES	50 (mM)	adjusted to pH6.54 with NaOH
4	1	drop	ammonium sulfate	200 (mM)
5	1	reservoir	ethanol	5.80 (%(v/v))
6	1	reservoir	HEPES	50 (mM)	adjusted to pH6.54 with NaOH
7	1	reservoir	ammonium sulfate	200 (mM)