1BFV
MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 89.900, 89.900, 59.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.187 * |
Rwork | 0.187 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nbv |
RMSD bond length | 0.011 |
RMSD bond angle | 0.037 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | AMoRE |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.074 * | |
Total number of observations | 129898 * | |
Number of reflections | 14834 | |
<I/σ(I)> | 9.53 | 3.1 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 8.8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | PROTEIN WAS CRYSTALLIZED FROM 18% (W/V) PEG 8000, 200MM ZN ACETATE AND 100MM NA CACODYLATE, PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-3 (mg/ml) | |
2 | 1 | drop | zinc acetate | 0.1 (M) | |
3 | 1 | drop | sodium cacodylate | 0.05 (M) | |
4 | 1 | drop | PEG8000 | 9 (%(w/v)) | |
5 | 1 | reservoir | zinc acetate | 0.2 (M) | |
6 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
7 | 1 | reservoir | PEG8000 | 18 (%(w/v)) |