1BFA
RECOMBINANT BIFUNCTIONAL HAGEMAN FACTOR/AMYLASE INHIBITOR FROM MAIZE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 57.120, 57.120, 80.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.26700 |
Structure solution method | DIRECT PHASING |
Starting model (for MR) | 1CBA |
RMSD bond length | 0.007 |
RMSD bond angle | 22.100 * |
Data reduction software | PROCESS |
Data scaling software | PROCESS |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 4996 | |
Completeness [%] | 77.0 | 45.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | PROTEIN WAS CRYSTALLIZED FROM 10MG/ML PROTEIN, 1% 2-PROPANOL, 1% PEG-4000, 0.1 M SODIUM CITRATE PH 5.4. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 30 (%) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |