1BEU
TRP SYNTHASE (D60N-IPP-SER) WITH K+
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 96 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.300, 59.700, 67.400 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27800 |
| Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1WSY |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 * | 0.281 * |
| Number of reflections | 49728 | |
| <I/σ(I)> | 16.6 | 5 |
| Completeness [%] | 87.0 | 63.3 |
| Redundancy | 4.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | Ahmed, S.A., (1985) J. Biol. Chem., 260, 3716. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Bicine | 50 (mM) | |
| 2 | 1 | drop | Na-EDTA | 1 (mM) | |
| 3 | 1 | drop | spermine | 0.8-1.5 (mM) | |
| 4 | 1 | drop | PEG8000 | 12 (%) |
