1BEO

BETA-CRYPTOGEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-06-22
Detector	MARRESEARCH
Spacegroup name	P 41 2 2
Unit cell lengths	46.510, 46.510, 134.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.218
Rwork	0.218
R-free	0.27900
Structure solution method	MAD+NATIVE DATA
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	MOSFLM
Phasing software	SHARP
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *
Rmerge	0.032 *	0.150 *
Total number of observations	35101 *
Number of reflections	7864
Completeness [%]	97.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	75 (mg/ml)
2	1	drop		0.02 (%)
3	1	drop		10 (mM)
4	1	reservoir		4.85 (M)
5	1	reservoir	sodium acetate	50 (mM)
6	1	reservoir	Tris-HCl	50 (mM)	can be replaced by 50mM CAPS