1BEN
INSULIN COMPLEXED WITH 4-HYDROXYBENZAMIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-02-14 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.720, 80.720, 37.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.154 * |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.034 |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.066 |
| Total number of observations | 159339 * |
| Number of reflections | 18002 |
| Completeness [%] | 76.6 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.7 | 0.05M SODIUM CITRATE, 0.007M ZINC ACETATE, 0.06M 4-HYDROXYBENZAMIDE, 1.0M SODIUM CHLORIDE, PH=5.7. CRYSTALS GROWN BY SLOW COOLING., slow cooling |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 0.9 (mM) | |
| 2 | 1 | 1 | sodium citrate | 0.05 (M) | |
| 3 | 1 | 1 | zinc acetate | 0.007 (M) | |
| 4 | 1 | 1 | 4-hydroxybenzamide | 0.06 (M) | |
| 5 | 1 | 1 | 1.0 (M) |
