1BEC

BETA CHAIN OF A T CELL ANTIGEN RECEPTOR

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Detector technology	IMAGE PLATE
Collection date	1994-04
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	100.600, 36.600, 71.500
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	7.000 - 1.700
R-factor	0.198
Rwork	0.198
RMSD bond length	0.013
RMSD bond angle	2.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.049	0.399 *
Total number of observations	60827 *
Number of reflections	25371 *
Completeness [%]	95.6 *	95.1 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.8 (mg/ml)
10	1	reservoir	ammonium sulfate	3 (%)
2	1	drop	Tris	7.3 (mM)
3	1	drop	HEPES	25 (mM)
4	1	drop		18.3 (mM)
5	1	dropdrop	PEG4000	1.0 (%(w/v))
6	1		ammonium sulfate	0.75 (%sat)
7	1	drop	agarose	0.014 (%)
8	1	reservoir	PEG4000	4 (%)
9	1	reservoir	HEPES	0.1 (M)