1BE3
CYTOCHROME BC1 COMPLEX FROM BOVINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 211.200, 211.200, 339.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| Rwork | 0.260 |
| R-free | 0.32000 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.266 |
| Total number of observations | 223182 * | |
| Number of reflections | 79396 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 88.9 | 76.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.6 | pH 6.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | n-dodecyl-beta-D-maltoside | 0.015 (%) | |
| 2 | 1 | 1 | 100 (mM) | ||
| 3 | 1 | 1 | PEG4000 | 6-8 (%) |
