1BCC
CYTOCHROME BC1 COMPLEX FROM CHICKEN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 169.590, 182.518, 240.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.160 |
| R-factor | 0.27 |
| Rwork | 0.270 |
| R-free | 0.31000 |
| Structure solution method | MIR AND NCS AVERAGING |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.270 | |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.095 | 0.324 |
| Total number of observations | 556456 * | |
| Number of reflections | 123869 | |
| <I/σ(I)> | 12.5 | 2.7 |
| Completeness [%] | 91.6 | 84.5 |
| Redundancy | 4.5 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 | 20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | KMES | 60 (mM) | pH6.7 |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | K-MOPS 7.5 | 20 (mM) | |
| 4 | 1 | drop | n-octyl-beta-D-glucopyranoside | 20 (g/l) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | reservoir | glycerol | 30 (%) |
