1BAM
STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.400, 46.000, 69.400 |
Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
Resolution | 10.000 * - 1.950 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.29700 * |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Number of reflections | 14335 |
Completeness [%] | 86.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.9 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
10 | 1 | reservoir | PEG8000 | 12 (%) | |
11 | 1 | reservoir | 20 (mM) | ||
12 | 1 | reservoir | glycerol | 10 (%) | |
13 | 1 | reservoir | 0.5 (M) | ||
2 | 1 | drop | 0.5 (M) | ||
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | DTT | 1 (mM) | |
5 | 1 | drop | glycerol | 15 (%) | |
6 | 1 | drop | PEG8000 | 12 (%) | |
7 | 1 | drop | 0.25 (M) | ||
8 | 1 | drop | 0.2 (%) | ||
9 | 1 | drop | crushed crystal |