1B9X
STRUCTURAL ANALYSIS OF PHOSDUCIN AND ITS PHOSPHORYLATION-REGULATED INTERACTION WITH TRANSDUCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-01-15 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.040, 89.330, 100.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.217 * |
Rwork | 0.217 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2trc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 13631 | |
<I/σ(I)> | 11.9 | 3.1 |
Completeness [%] | 98.4 | 98.2 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CITRATE | ||
2 | 1 | 1 | MAGNESIUM ACETATE | ||
3 | 1 | 1 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | DTT | 0.5 (mM) | |
3 | 1 | drop | sodium citrate | 25 (mM) | |
4 | 1 | drop | magnesium acetate | 75 (mM) | |
5 | 1 | drop | PEG8000 | 4.75 (%) | |
6 | 1 | reservoir | sodium citrate | 50 (mM) | |
7 | 1 | reservoir | magnesium acetate | 150 (mM) | |
8 | 1 | reservoir | PEG8000 | 9.5 (%) |