1B8K

Neurotrophin-3 from Human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 21 21 2
Unit cell lengths	38.300, 52.300, 67.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.150
R-factor	0.238
Rwork	0.238
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bnf
RMSD bond length	0.015
RMSD bond angle	2.150
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.150
Rmerge	0.047
Number of reflections	7414
Completeness [%]	82.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	15 *	CRYSTALLIZATION CONDITIONS: 7% ISOPROPANOL 185 MM NACITRATE PH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG8000	22 (%)
4	1	reservoir	PIPES	100 (mM)
5	1	drop	24 amino acid peptide		1:2 ratio (NT4 protomer:peptide)