1B8H
SLIDING CLAMP, DNA POLYMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.000, 90.500, 146.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.271 * |
| Rwork | 0.265 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b77 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.105 * |
| Total number of observations | 47361 * |
| Number of reflections | 18527 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 92.6 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 20 * | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | calcium acetate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 8-9 (%(w/v)) | |
| 3 | 1 | drop | calcium acetate | 0.1 (M) | |
| 4 | 1 | drop | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | PEG8000 | 16-18 (%(w/v)) | |
| 6 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) |
