1B8G

1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-03-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	53.630, 69.060, 123.240
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	19.700 - 2.370
Rwork	0.179
R-free	0.24200
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	25.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.430
High resolution limit [Å]	2.370	2.370
Rmerge	0.098 *	0.104 *
Number of reflections	34176
<I/σ(I)>	9.8	6.2
Completeness [%]	93.6	80.8
Redundancy	1.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	dithiothreitol	0.2 (mM)
3	1	drop	PLP	0.01 (mM)
4	1	drop		0.01 (%(w/v))
5	1	drop	HEPES	30 (mM)
6	1	reservoir	PEG4000	18-24 (%(w/v))
7	1	reservoir	MES	50 (mM)