1B7T

MYOSIN DIGESTED BY PAPAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-01-15
Spacegroup name	P 1
Unit cell lengths	51.700, 58.900, 138.900
Unit cell angles	89.10, 90.00, 73.50

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.2244 *
Rwork	0.224
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mmd 1wdc
RMSD bond length	0.010
RMSD bond angle	25.910 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (4.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.048	0.110 *
Number of reflections	43838	5968 *
<I/σ(I)>	11.6
Completeness [%]	80.6	38.5 *
Redundancy	3	1.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	4 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG20000	3 (%)
2	1	drop	Tris	50 (mM)
3	1	drop	glycerol	6 (%)
4	1	drop		40 (mM)
5	1	drop	protain	10 (mg/ml)
6	1	drop	MgADP	2 (mM)