1B7G
GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.550, 101.550, 179.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 - 2.050 |
| R-factor | 0.229 * |
| Rwork | 0.226 |
| R-free | 0.29300 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.056 | 0.472 |
| Number of reflections | 59606 | |
| <I/σ(I)> | 27.3 | 2.2 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 5.9 | 2.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 18 (%(w/v)) | |
| 3 | 1 | drop | PIPES | 10 (mM) | |
| 4 | 1 | drop | EDTA | 5 (mM) | |
| 5 | 1 | drop | NAD+ | 10 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 35 (%) | |
| 7 | 1 | reservoir | PIPES | 10 (mM) |
