1B6S
STRUCTURE OF N5-CARBOXYAMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 280 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-10 |
| Detector | BRUKER AXS |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.600, 92.100, 102.600 |
| Unit cell angles | 66.10, 82.70, 81.80 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| Rwork | 0.185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b6r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 19.300 * |
| Data reduction software | XCALIBRE |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.181 |
| Number of reflections | 66345 | |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 95.7 | 89.6 |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | HEPES | 100 (mM) | |
| 3 | 1 | 1 | 125 (mM) | ||
| 4 | 1 | 1 | 5.0 (mM) | ||
| 5 | 1 | 1 | AMP-PNP | 5 (mM) | |
| 6 | 1 | 1 | PEG | 8-17 (%(w/v)) |
