1B6Q
ALANINE 31 PROLINE MUTANT OF ROP PROTEIN
Experimental procedure
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.400, 42.100, 81.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.825 - 1.800 |
R-factor | 0.188 * |
Rwork | 0.189 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | POLY-ALANINE MODEL FROM PDB ENTRY 1ROP |
RMSD bond length | 0.007 |
RMSD bond angle | 0.909 |
Data reduction software | CAD4 |
Data scaling software | CAD4 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.825 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 5103 | |
<I/σ(I)> | 12.4 | 2.48 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.3 | pH 4.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium acetate | 100 (mM) | |
3 | 1 | drop | ammonium sulfate | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) |