1B5M
RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1996-04-18 |
Detector | SIEMENS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 30.000, 30.000, 219.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.22 * |
Rwork | 0.220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3B5C |
RMSD bond length | 0.016 |
RMSD bond angle | 2.900 |
Data reduction software | FRAMBO |
Data scaling software | SAINT |
Phasing software | MRCHK |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.050 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.049 * | 0.088 * |
Number of reflections | 3123 | |
<I/σ(I)> | 1.5 | 1 |
Completeness [%] | 96.0 | 85 |
Redundancy | 2.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION TOGETHER WITH SLOW EVAPORATION USING 20% PEG 8000 IN 0.1M SODIUM CACODYLATE (PH = 6.5 WITH 0.2 M MAGNESIUM ACETATE., vapor diffusion with slow evaporation |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 20 (%) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
4 | 1 | reservoir | magnesium acetate | 0.2 (M) |