1B5E
DCMP HYDROXYMETHYLASE FROM T4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-15 |
Detector | FUJI |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.220, 53.120, 75.170 |
Unit cell angles | 90.00, 115.29, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.600 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.20800 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 0.921 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 80033 |
Completeness [%] | 97.3 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.73 * | Song, S.H., (1999) Acta Crystallogr. D55, 1061. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | dCMP | ||
3 | 1 | reservoir | Tris-HCl | 100 (0.9) | |
4 | 1 | reservoir | sodium citrate | 0.9 (M) | pH8.73 |