1B4L
15 ATMOSPHERE OXYGEN YEAST CU/ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298K) STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.900, 118.900, 75.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 * | |
| Total number of observations | 64930 * | |
| Number of reflections | 16745 | |
| <I/σ(I)> | 22 | 7.4 |
| Completeness [%] | 87.7 | 90.5 |
| Redundancy | 3.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.7 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15-25 (mg/ml) | |
| 2 | 1 | 1 | phosphate | 50 (mM) | |
| 3 | 1 | 2 | ammonium sulfate | 2.0-2.2 (M) | |
| 4 | 1 | 2 | Tris-HCl | 50 (mM) | |
| 5 | 1 | 2 | 50 (mM) | ||
| 6 | 1 | 2 | 0.001 (%(w/v)) |
