1B3J
STRUCTURE OF THE MHC CLASS I HOMOLOG MIC-A, A GAMMADELTA T CELL LIGAND
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05 |
Detector | RIGAKU RAXIS VI |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 261.500, 261.500, 261.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9999.000 * - 2.800* |
R-factor | 0.253 * |
Rwork | 0.246 |
R-free | 0.29300 * |
Structure solution method | MIR |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.050 |
High resolution limit [Å] | 2.800 * | 3.000 |
Rmerge | 0.063 * | 0.329 * |
Total number of observations | 130489 * | |
Number of reflections | 19368 * | |
<I/σ(I)> | 23.5 | 3.2 |
Completeness [%] | 99.7 * | 100 * |
Redundancy | 4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | 291 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | CITRATE BUFFER | ||
3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
2 | 1 | reservoir | sodium citrate | 50-100 (mM) |