1B2W
COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 286 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.100, 74.600, 104.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.33100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.200 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.960 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.093 | 0.410 |
| Number of reflections | 11491 | |
| <I/σ(I)> | 8.3 | 1 |
| Completeness [%] | 67.2 * | 0.28 |
| Redundancy | 2.2 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.2 | 18-20 * | 6.6% PEG 8000(W/V), IN 0.05 M NACL AND 0.05% SODIUM AZIDE., pH 6.2 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 2 | 6.6% PEG 8000(W/V) | ||
| 2 | 1 | 2 | 0.05 M NACL | ||
| 3 | 1 | 2 | 0.05% SODIUM AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35-45 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 6.6 (%(w/v)) | |
| 3 | 1 | reservoir | 0.05 (M) | ||
| 4 | 1 | reservoir | sodium azide | 0.05 (%) |
