1B2O
CLOSTRIDIUM PASTEURIANUM RUBREDOXIN G10VG43A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-01 |
| Detector | RIGAKU RU200 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.850, 61.850, 80.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5rxn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 1.900 | 1.940 |
| Rmerge | 0.092 | 0.280 |
| Total number of observations | 54730 * | |
| Number of reflections | 12136 | |
| <I/σ(I)> | 10.9 | 3.7 |
| Completeness [%] | 94.0 | 96 * |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 277 * | PROTEIN WAS CRYSTALLISED FROM 70% SATURATED AMMONIUM SULFATE IN SODIUM ACETATE BUFFER (50 MM) AT PH 5.0. |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 2 | 70% SATURATED AMMONIUM SULFATE | ||
| 2 | 1 | 2 | SODIUM ACETATE BUFFER (50 MM) AT PH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2-35 (%) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 50-70 (%) | |
| 5 | 1 | reservoir | sodium acetate | 50 (mM) |
