1AZT
GS-ALPHA COMPLEXED WITH GTP-GAMMA-S
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-06 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.300, 96.500, 133.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gia |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.540 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.138 * | |
| Number of reflections | 37636 | |
| <I/σ(I)> | 8.3 | 2.5 |
| Completeness [%] | 94.0 | 95.1 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | CRYSTALLIZED IN HANGING DROPS CONTAINING PROTEIN MIXED 1:1 WITH WELL SOLUTION CONSISTING OF 90-100% SATURATED KH2PO4 OR NAH2PO4 (UNBUFFERED)., pH 4.5, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Gsalpha | 12 (mg/ml) | |
| 2 | 1 | drop | GTPgammaS | 0.8 (mM) | |
| 3 | 1 | drop | sodium HEPES | 20 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 5 (mM) | |
| 7 | 1 | reservoir | potassium phosphate | 90-100 (%sat) | can be replaced by 2.5M sodium phosphate |
