1AZS
COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-06 |
| Detector | ADSC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.000, 134.050, 70.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gia |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.340 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 * | |
| Number of reflections | 37320 | |
| <I/σ(I)> | 11.5 | 2.5 |
| Completeness [%] | 75.1 | 46.6 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | CRYSTALLIZED IN HANGING DROPS CONTAINING PROTEIN MIXED 1:1 WITH WELL SOLUTION OF 7.2-7.5% PEG 8000, 500MM NACL AND 100 MM (PH 5.4-5.6), vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 5 (mM) | |
| 2 | 1 | drop | MPFsk | 0.200 (mM) | |
| 3 | 1 | drop | GTPgammaS | 0.500 (mM) | |
| 4 | 1 | drop | complex | 8 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 7.2-7.5 (%) | |
| 6 | 1 | reservoir | 500 (mM) | ||
| 7 | 1 | reservoir | MES | 100 (mM) | pH5.4-5.6 |
