1AYZ
CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE UBIQUITIN-CONJUGATING ENZYME RAD6 (UBC2) AT 2.6A RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 113.750, 146.360, 109.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.24600 |
| Structure solution method | SIR AND MOL REP TO PERFORM THREE-FOLD AVERAGING OF SIR MAP |
| Starting model (for MR) | 1AAK |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 * | 0.269 * |
| Total number of observations | 76204 * | |
| Number of reflections | 26012 | |
| <I/σ(I)> | 12.9 | 3.6 |
| Completeness [%] | 91.3 | 66.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 12 (%(w/w)) | |
| 2 | 1 | reservoir | MES | 50 (mM) | pH5.0 |
| 3 | 1 | reservoir | spermine tetrahydrochloride | 1 (%(w/v)) | |
| 4 | 1 | drop | protein | 30 (mM) |
