1AYM

HUMAN RHINOVIRUS 16 COAT PROTEIN AT HIGH RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-04
Detector	FUJI
Spacegroup name	P 2 21 21
Unit cell lengths	362.600, 347.100, 334.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.150
R-factor	0.23
Rwork	0.230
R-free	0.23300
Structure solution method	MOLECULAR
Starting model (for MR)	2RHN
RMSD bond length	0.010
RMSD bond angle	26.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	1.900	2.140
Rmerge	0.097	0.240
Total number of observations	3442752 *
Number of reflections	1452910
Completeness [%]	44.5	47
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	4-5 (mg/ml)
2	1	drop	PEG8000	0.25-0.75 (%)
3	1	reservoir	PEG8000	0.5-1.5 (%)
4	1	reservoir		5-20 (mM)