1AYA
CRYSTAL STRUCTURES OF PEPTIDE COMPLEXES OF THE AMINO-TERMINAL SH2 DOMAIN OF THE SYP TYROSINE PHOSPHATASE
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.800, 52.700, 56.400 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.050 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.050 * |
| Rmerge | 0.084 * |
| Number of reflections | 11145 * |
| Completeness [%] | 90.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | protein | ||
| 2 | 1 | reservoir | peptide | ||
| 3 | 1 | reservoir | PEG4000 | 15 (%) | |
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | protein |
