1AY5
AROMATIC AMINO ACID AMINOTRANSFERASE COMPLEX WITH MALEATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-08 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 124.480, 121.440, 55.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1art |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.000 * |
| Data reduction software | PROCESS ((RIGAKU)) |
| Data scaling software | PROCESS ((RIGAKU)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.000 | 2.800 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.145 |
| Total number of observations | 86526 * | |
| Number of reflections | 26633 | |
| <I/σ(I)> | 14.1 | 5.2 |
| Completeness [%] | 97.2 | 96.1 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 0.05 (mM) | |
| 4 | 1 | drop | 0.01 (%) | ||
| 5 | 1 | drop | PEG4000 | 8.25 (%(w/v)) | |
| 6 | 1 | drop | sodium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG4000 | 16.5 (%(w/v)) | |
| 8 | 1 | reservoir | sodium acetate | 0.4 (M) |
