1AY4
AROMATIC AMINO ACID AMINOTRANSFERASE WITHOUT SUBSTRATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-07 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.720, 123.060, 55.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.330 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1art |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.300 * |
| Data reduction software | PROCESS ((RIGAKU)) |
| Data scaling software | PROCESS ((RIGAKU)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.000 | 2.500 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.075 | 0.174 |
| Total number of observations | 110551 * | |
| Number of reflections | 33010 | |
| <I/σ(I)> | 11.5 | 4 |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.7 | 20 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 0.05 (mM) | |
| 4 | 1 | drop | 0.01 (%) | ||
| 5 | 1 | drop | PEG4000 | 8.25 (%(w/v)) | |
| 6 | 1 | drop | sodium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG4000 | 16.5 (%(w/v)) | |
| 8 | 1 | reservoir | sodium acetate | 0.4 (M) |
