1AXD
STRUCTURE OF GLUTATHIONE S-TRANSFERASE-I BOUND WITH THE LIGAND LACTOYLGLUTATHIONE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.540, 78.910, 55.610 |
| Unit cell angles | 90.00, 107.24, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.21600 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.600 * |
| Data reduction software | CCP4 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.089 |
| Number of reflections | 13788 |
| Completeness [%] | 92.0 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG | 22 (%) | |
| 2 | 1 | drop | magnesium acetate | 0.3 (M) | |
| 3 | 1 | drop | MES | 0.1 (M) | |
| 4 | 1 | drop | lactoylglutathione | 5 (mM) |
