1AX9

ACETYLCHOLINESTERASE COMPLEXED WITH EDROPHONIUM, LAUE DATA

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	273
Detector technology	CCD
Collection date	1995-04-30
Spacegroup name	P 31 2 1
Unit cell lengths	113.000, 113.000, 136.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.207
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ace
Data reduction software	LaueView
Data scaling software	LaueView
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.200	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.148 *
Total number of observations	83171 *
Number of reflections	21492
Completeness [%]	84.5	61.3
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	4 *	Raves, M.L., (1997) Nature Struct.Biol., 4, 57. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG200	38 (%)
2	1	reservoir	MES	0.1 (M)
3	1	drop	protein	12 (mg/ml)