1AW9

STRUCTURE OF GLUTATHIONE S-TRANSFERASE III IN APO FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1995-03
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	97.770, 97.770, 116.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.197
Rwork	0.197
R-free	0.22400 *
RMSD bond length	0.014
RMSD bond angle	22.800 *
Data reduction software	CCP4
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	1.970
Rmerge	0.087
Number of reflections	15788
Completeness [%]	99.5
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	drop	sodium azide	0.02 (%)
4	1	reservoir		70 (mM)
5	1	reservoir	sodium acetate	1.0 (M)
6	1	reservoir	HEPES	0.1 (M)	pH7.5
7	1	reservoir	sodium azide	0.02 (%)