1AW8

PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	293
Collection date	1996-07-31
Spacegroup name	P 61 2 2
Unit cell lengths	72.170, 72.170, 216.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.2
Rwork	0.200
R-free	0.23900
Structure solution method	MIR
RMSD bond length	0.008 *
RMSD bond angle	1.468 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.078	0.191
Number of reflections	16129
<I/σ(I)>	6.4	3.6
Completeness [%]	98.0	90 *
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *		PROTEIN WAS CRYSTALLIZED FROM 12% PEG 2000 MME, 0.1 M NA ACETATE, PH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG MME2000	12 (%(w/v))
4	1	reservoir	sodium acetate	0.1 (M)