1AVZ

V-1 NEF PROTEIN IN COMPLEX WITH WILD TYPE FYN SH3 DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	100
Detector technology	CCD
Collection date	1996-10-04
Detector	PRINCETON 2K
Spacegroup name	P 65 2 2
Unit cell lengths	108.200, 108.200, 223.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.22
Rwork	0.220
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1EFN AND 1SHF
RMSD bond length	0.010
RMSD bond angle	24.100 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	3.000
Rmerge	0.067
Total number of observations	57175 *
Number of reflections	14778
Completeness [%]	91.0
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.9		unpublished data *