1AVQ

TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1997-01
Spacegroup name	P 41 21 2
Unit cell lengths	156.700, 156.700, 131.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.198
Structure solution method	MIR WITH ANOMALOUS DISPERSION
RMSD bond length	0.017
RMSD bond angle	17.950 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.055 *
Total number of observations	291671 *
Number of reflections	62855
<I/σ(I)>	11.9	4.7
Completeness [%]	97.0	94.3
Redundancy	4.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.7	277	PROTEIN WAS CRYSTALLIZED FROM 1.2 M NH2SO4, 0.2 M NACL 0.1 M NAACETATE PH 4.7, AT 4 DEGREES CELSIUS., temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium salfate	1.2 (M)
2	1	1		0.2 (M)
3	1	1	sodium acetate	0.1 (M)