1AV8

RIBONUCLEOTIDE REDUCTASE R2 SUBUNIT FROM E. COLI

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1996-08
Spacegroup name	P 61
Unit cell lengths	139.700, 139.700, 110.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.800
R-factor	0.139
Rwork	0.139
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rib
RMSD bond length	0.019
RMSD bond angle	20.880 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.920
High resolution limit [Å]	2.800	2.800
Rmerge	0.089 *
Number of reflections	299329
<I/σ(I)>	11.3	10
Completeness [%]	99.0	92 *
Redundancy	5.5	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.6		PROTEIN WAS CRYSTALLIZED FROM 80% SATURATED NACL, NEAR PHYSIOLOGICAL PH (PH 7.6)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop	glycerol	5 (%)
4	1	reservoir		80 (%sat)