1AUI

HUMAN CALCINEURIN HETERODIMER

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	87
Detector technology	IMAGE PLATE
Collection date	1994-10
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	48.810, 104.340, 177.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.187
Rwork	0.187
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT PLUS ANOMALOUS SCATTERING
RMSD bond length	0.019
RMSD bond angle	23.320 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	XTALVIEW

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Rmerge	0.069 *
Number of reflections	50007
Completeness [%]	92.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		PROTEIN WAS CRYSTALLIZED FROM 8% PEG 6000, 0.1M CACL2, 0.1M TES PH 7.5, 1 MM DTT.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG6000