1ATT

CRYSTAL STRUCTURE OF CLEAVED BOVINE ANTITHROMBIN III AT 3.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	91.300, 91.300, 383.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.200
R-factor	0.212
Rwork	0.212
RMSD bond length	0.015
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	41.000 *
High resolution limit [Å]	3.200 *
Rmerge	0.091 *
Total number of observations	61000 *
Number of reflections	23436 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	13 (mg/ml)
2	1	2	ammonium sulphate	70 (%sat)
3	1	2	potassium	30 (mM)
4	1	2		1 (mM)	can be replaced with HgCl2, ZnCl2
5	1	2		0.01 (%(w/v))