1AT0
17-kDA fragment of hedgehog C-terminal autoprocessing domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-15 |
| Detector | FUJI |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 101.540, 101.540, 101.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.2176 |
| Rwork | 0.218 |
| R-free | 0.27540 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.382 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 * | |
| Number of reflections | 26790 | |
| <I/σ(I)> | 19.9 | 4.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 20% PEG 3350, 80 mM AMMONIUM SULFATE, 10 mM SODIUM CACODYLATE pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Hh-C17 | 1.4 (mg/ml) | |
| 2 | 1 | drop | beta-mercaptoethanol | 1.4 (mM) | |
| 3 | 1 | reservoir | PEG3350 | 20 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 80 (mM) | |
| 5 | 1 | reservoir | sodium cacodylate | 10 (mM) |
