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X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAL 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN OPEN AND CLOSED FORM

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	157.140, 85.510, 78.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.800
R-factor	0.217
Rwork	0.217
RMSD bond length	0.017
RMSD bond angle	3.300 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.045 *
Total number of observations	105688 *
Number of reflections	31930 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	0.9 (mM)
10	1	reservoir	sodium sulfate	1 (M)
2	1	drop	potassium phosphate	10 (mM)
3	1	drop	PLP	0.01 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop		0.3 (mM)
6	1	reservoir	potassium phosphate	10 (mM)
7	1	reservoir	PLP	0.01 (mM)
8	1	reservoir	EDTA	1 (mM)
9	1	reservoir		0.3 (mM)