1AQM

ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	71.400, 138.900, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 1.850
R-factor	0.157
Rwork	0.157
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PORCINE PANCREAS ALPHA-AMYLASE (QIAN ET AL. J.MOL.BIOL. 231 785-799 (1993))
RMSD bond length	0.008
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	Agrovata
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.085 *	0.499 *
Total number of observations	153351 *
Number of reflections	42049
<I/σ(I)>	8.3	1.5
Completeness [%]	85.9	61.8
Redundancy	3.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4, 20 *	prtein to mother liquor ration was 2:1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	60-70 (%)
2	1	reservoir	HEPES	0.1 (M)