1AQL
CRYSTAL STRUCTURE OF BOVINE BILE-SALT ACTIVATED LIPASE COMPLEXED WITH TAUROCHOLATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-03 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.230, 104.090, 120.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1akn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.920 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.117 * | 0.206 * |
| Number of reflections | 33877 | |
| <I/σ(I)> | 9.1 | 2.4 |
| Completeness [%] | 82.7 | 48.3 |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 3 | 1 | drop | isopropanol | 2.5 (%) | |
| 4 | 1 | drop | TC | 0.5 (mM) | |
| 5 | 1 | drop | sucrose monolaurate | 0.05 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 7 | 1 | reservoir | isopropanol | 5 (%) | |
| 8 | 1 | reservoir | TC | 1 (mM) | |
| 9 | 1 | reservoir | sucrose monolaurate | 0.1 (mM) |
