1AQB

RETINOL-BINDING PROTEIN (RBP) FROM PIG PLASMA

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	1996-09
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	45.810, 53.137, 72.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.000 - 1.650
R-factor	0.1844
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hbp
RMSD bond length	0.030
RMSD bond angle	0.030
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	1.720
High resolution limit [Å]	1.650	1.650
Rmerge	0.064	0.190
Total number of observations	84968 *
Number of reflections	20127
<I/σ(I)>	8	4
Completeness [%]	91.0	56
Redundancy	4.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277 *	SITTING DROP VAPOR DIFFUSION METHOD, AT A FINAL PROTEIN CONCENTRATION OF 8 MG/ML IN THE PRESENCE OF 8% 2-METHYL-2,4-PENTANEDIOL, 3 MM CADMIUM ACETATE, 0.1 M TRIS-ACETATE, PH=6.8, vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	MPD	8 (%(v/v))
3	1	reservoir	cadmium acetate	3 (mM)
4	1	reservoir	Tris-acetate	0.1 (M)