1AQ2
PHOSPHOENOLPYRUVATE CARBOXYKINASE
Experimental procedure
| Source details | ENRAF-NONIUS |
| Temperature [K] | 300 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-10-15 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.190, 96.610, 46.860 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 |
| Data reduction software | MADNES |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 39324 | |
| <I/σ(I)> | 4.3 | 2.1 |
| Completeness [%] | 90.0 | 85 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.4 | pH 4.4 |
