1AO5

MOUSE GLANDULAR KALLIKREIN-13 (PRORENIN CONVERTING ENZYME)

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	73.750, 73.750, 192.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.330 - 2.600
R-factor	0.197
Rwork	0.197
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kai
RMSD bond length	0.008
RMSD bond angle	24.808 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.330	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.065	0.345
Number of reflections	17034
<I/σ(I)>	9.6	1.7
Completeness [%]	95.3	86.8
Redundancy	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		MGK-13 (57 MG/ML) WAS CRYSTALLIZED USING 10-15% PEG8000, 0.2M LISO4, 0.1M SODIUM CACODYLATE, PH 6.5 BY HANGING DROP VAPOR DIFFUSION METHOD., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	mGK-13	57 (mg/ml)
2	1	reservoir	PEG8000	10-15 (%)
3	1	reservoir		200 (mM)
4	1	reservoir	sodium cacodylate	100 (mM)