1AO3

A3 DOMAIN OF VON WILLEBRAND FACTOR

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	58.000, 65.100, 57.800
Unit cell angles	90.00, 101.90, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.158
Rwork	0.158
R-free	0.21500
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.056 *
Number of reflections	15112 *
<I/σ(I)>	11.1	4
Completeness [%]	95.5	93.3
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	22 *	0.1M TRIS PH 8.5, 200MM MGCL2, 24% PEG 8K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	24-32 (%)
2	1	reservoir	Tris	100 (mM)
3	1	reservoir		200 (mM)